Figure 4
Metabolic inhibitors effectively promote tumor regression . HCT116 cells (107 cells per animal) with low bioenergetic signature (G-cells) were injected into nude mice for tumor development. Twenty days after, when tumor volume reached ~1 cm3, the animals received daily 100 μL intraperitoneal injections, containing 8 μM 3BrP (n = 10, open square), 0.5 mM 5-FU (n = 5, grey square) or 100 μM IA (n = 7, closed square) for six consecutive days. A 0.9% NaCl-treated control group (n = 6, hatched square) was also included for comparison. (A) Tumor volume is presented normalized to its volume before initiation of the treatments. * and #, p < 0.05 when comparing 3BrP with 5-FU- and IA-treated mice, respectively. **, p < 0.05 for multiple comparisons by ANOVA. Inserts provide representative examples of the differences in tumor size compared to controls. (B) Mice body weight (g) after treatments. Results shown are means ± SEM. *, p < 0.05 when compared to NaCl-treated controls by Student's t test.