The Bioenergetic Signature correlates with the cell-death response to chemotherapy . HCT116 cells were treated as indicated  to produce cells with low (G-cells) (A), medium (M-cells) (B) and high (SM-cells) (C) bioenergetic signatures (β-F1/GAPDH ratio). The rates of aerobic glycolysis in G-, M- and SM-cells are also indicated. Cells were exposed to the following agents: 8 μM 3BrP, 100 μM IA, 10 μM 5-FU or were left untreated (Control). Cells were double-stained with Hoechst 33342 and propidium iodide and visualized using fluorescence microscopy at 20x magnification. The percentage of dead cells (red cells, PI positive) was determined by examination of different randomly selected fields. Histograms shown (A-C) represent the means ± SEM of 10-25, 10-24 and 10-23 independent determinations in G-, M-, and SM-cells respectively. *, P < 0.05 for multiple comparisons by ANOVA and post hoc Dunnett's test. Plots in (D) illustrate the inverse (3BrP and IA) and direct (5-FU) correlation that exists between the bioenergetic signature of the cells and the death-response to the chemotherapeutic agents.