Figure 4From: Huh-7 cell line as an alternative cultural model for the production of human like erythropoietin (EPO)Intra and extra-cellular expression analysis of EPO with and without Kozak sequence addition, through ELISA. Optical density was measured at 450 nm. Both intra and extra-cellular EPO expression increased after the addition of Kozak sequence. (A) Comparison of extra-cellular EPO expression with Kozak (pcDNA3.1-K-SS-EPO) and without Kozak sequence (pcDNA3.1-SS-EPO) expression vectors. (B) Comparison of intra-cellular EPO expression after the addition of Kozak.Back to article page