PCSD1 xenograft tumors express luminal-type epithelial, advanced prostate cancer biomarkers. A. RT-PCR analysis was performed on cDNA synthesized with reverse transcriptase (RT+) or without (RT-) from RNA purified from PCSD1 sub-cutaneous xenograft tumors, cultures of LAPC4, a human prostate cancer cell line and K562, a human CML cell line culture, as well as murine spleen, bone marrow and liver or H2O alone and RNA from normal human prostate tissue. Human specific primers for keratins 5, 8, 14 and 18, AMACR and NKX3.1, GAPDH and mouse-specific GAPDH were used to detect expression of these genes. B. RT-PCR analysis was performed on intra-femoral xenograft PCSD1 tumor cells, cultured VCaP prostate cancer cell line as well as normal human prostate tissue. Primers were specific for RNA from full length human AR or the TMPRSS2-ERG fusion gene. GAPDH levels were comparable.