Figure 5

IFN-γ Secreting T Cells was measured by ELISPOT assay. ELISPOT assay counting IFN-γ secreting T cells was performed on before (Pre: White bar) and after the vaccination (Post: Black bar). PBMCs were incubated with KLH for 20 h at 37°C to induce the Ag-specific IFN-γ secretion. Stars are indicated the statistical significance (*p < 0.05) comparing before vs. after vaccination.