From: Tumour macrophages as potential targets of bisphosphonates
Cell Type | Bisphosphonate | Main Findings | Reference |
---|---|---|---|
J774 cells RAW 264 cells | PAM, ALN, IBA, 100 μM 24 hours | Inhibited macrophage proliferation. Reduced cell viability. Increased cell death. | Rogers et al [68] |
J774 cells | ALN, 25 μM or 100 μM IBA 5 μM, 7.5 μM or 10 μM 24 hours | Dose dependent increase in accumulation of unprenylated Rap1A. | Firth et al [97] |
RAW 264 cells | ALN 10 μM; 5, 7, 9 or 16 hours ALN 100 μM; 1, 3, 5, 7, 9, 16 hours. | Dose and time dependent increase in accumulation of unprenylated Rap1A. Detectable after 16 hours incubation with 10 μM or 5 hours incubation with 100 μM | Monkkonen et al [98] |
J774 A.1 cells | PAM, ZOL, ALN, RIS 1-100 μM for 72 hours. | All BPs induced significant apoptosis ZOL > RIS > ALN > PAM. | Moreau et al [49] |
Macrophage precursor from bone marrow cells and bone marrow derived macrophages | PAM 2.5 × 10-7M (=0.25 μM), 96 hours | Significant inhibition M-CSF induced proliferation of bone marrow precursors | Cecchini et al [69] |
Activated human monocyte/macrophage | PAM 100-300 μM 24 hour pre-treatment Then activated with LPS. | Dose-dependent inhibition of MMP-9 expression Lower doses PAM increases expression. | Valleala et al [71] |
Human macrophage-like cell line U937 | Clodrolip 20-200 μM 2 days | Decreased cell survival | Hiraoka et al [85] |
Murine Macrophages | Clodrolip 9 μg per well 29 hours | Decreased cell viability | Gazzangia et al [86] |
Murine peritoneal macrophages | Clodrolip 1 mg/ml 6 hours. | Dose-dependent increase in apoptosis. | Zeisberger et al [87] |
Bone marrow cells from naive mice cultured with M-CSF or tumour supernatant. | Zoledronic acid 0.03, 0.15, 0.3 μM 6 days | Dose-dependent inhibition in differentiation of myeloid cells to macrophages. Decreased in M2 phenotype compared to control. | Veltman et al [89] |