Fenofibrate upregulates NF- kappaB and iNOS expression in the pancreas in obese MSG rats. Quantitative RT-PCR analysis of pancreatic NF- kappaB and iNOS mRNA levels (A), UCP2 and PPAR alpha mRNA levels (B) in the three groups. A comparative threshold cycle (CT) method was used for relative quantification of gene expression using β-actin for normalization. Measurements were carried out in triplicate for each sample. (C) Western blot analysis of pancreatic NF- kappaB p65, iNOS and TNF alpha protein levels in three groups. Beta actin served as loading control. Data represented the mean of at least three independent experiments ± s.e.m. *p < 0.05, ** p < 0.01 vs. control.