Two plates run together compared for A) IL-6 and B) IL-8 values. A set of 38 cell culture samples were run on both an 8-plex and a 10-plex plate. The values for IL-6 and IL-8 are compared on a log scale. Each plate had a unique upper limit. The values for IL-6 show excellent concordance, and the 8-plex upper limit was 1.8 times the upper limit. The IL-8 values were reproducibly higher (1.25×) in the 10-plex plate where the upper limit was 2.4 times higher.