Figure 6

Effect of pIgA, IgA-HMC conditioned medium, AngII and Aldo on the expression of MR or AT2R. Growth arrested PTEC was cultured with (i) pIgA (IgA, 0.5 mg/ml) from IgAN patients; (ii) 4 fold diluted IgA-HMC medium prepared from controls or IgAN patients; (iii) Aldo (10^-10 M) or (iv) AngII (10^-10 M). Both the exogenous AngII and IgA-HMC medium prepared from IgAN patients significantly increased the expression of (A) MR mRNA, (B) MR protein, (C) AT2R mRNA and (D) AT2R protein as compared with PTEC cultured with plain medium control (** signifies p < 0.01). The results represent the mean ± SD from five individual experiments.