Figure 3From: Replication efficiency of oncolytic vaccinia virus in cell cultures prognosticates the virulence and antitumor efficacy in miceForeign gene expression cassette removal derivatives kill tumor cells in culture more efficiently than their parental virus GLV-1h68. GI-101A cells were infected with each virus strain at an MOI of 0.01. Cell viability was determined using CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega, Madison, WI, USA). Cell survival before infection was set at 100%. The values are the mean of quadruplicate samples, and the bars indicate SD. The data represents two independent experiments. Statistical analysis was performed using two-way ANOVA. *, **, and *** indicate P < 0.05, 0.01, and 0.001, respectively, when compared with the GLV-1h68 group.Back to article page