Figure 5
The demethylating agent 5-aza-2-deoxycytidine restores SLC22A18 expression in U251 cells and suppresses cell growth. (A) Western blotting indicating a significant increase in SLC22A18 expression after treatment of U251 cells with 2 μg/ml 5-aza-2-deoxycytidine for 7 days: 1, control cells; 2, treated cells; ß-actin was used as an internal control. (B) Growth curves demonstrating reduced cell numbers in the 5-aza-2-deoxycytidine-treated group, compared to empty plasmid transfected U251-EV cells and untransfected U251 cells.