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Table 2 Cocktails for DC maturation

From: Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission

Cocktail

Inflammatory Cytokines/IFN

Other Additives

TLR Ligands

C

TNF-α, IL-1β, IFN-γ

PGE2

CL075

CP

TNF-α, IL-1β, IFN-γ

PGE2

CL075, poly(I:C)

R

TNF-α, IL-1β, IFN-γ

PGE2

R848

RP

TNF-α, IL-1β, IFN-γ

PGE2

R848, poly(I:C)

  1. Following concentrations were used in the individual maturation cocktails:
  2. All cocktails contained 1100 IU/mL TNF-α, 2000 IU/mL IL-1β, 5000 U/ml IFN-γ and 250 ng/ml PGE2.
  3. Additional additives as follows:
  4. Cocktail C: 1 μg/ml CL075
  5. Cocktail CP: 1 μg/ml CL075 and 20 ng/ml poly (I:C)
  6. Cocktail R: 1 μg/ml R848
  7. Cocktail RP: 1 μg/ml R848 and 20 ng/ml poly (I:C)