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Figure 6 | Journal of Translational Medicine

Figure 6

From: Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission

Figure 6

Induction of CMVpp65 and WT-1 specific T-cell response with peptide loaded 3-day DCs. (A) Percentage of CMVpp65- and WT1-specific CD8 T cells was determined by staining with anti-CD8 and the appropriate HLA-matching multimeric complex. As a negative control, a tetramer specific for an HIV-specific peptide was included. Gates were set on the lymphocyte population and on CD3 positive cells. Results are given as % CD8/tetramer double-positive cells. DCs used for initial stimulation were generated using the RP cocktail. One representative experiment is shown. (B) The functional activity of the CTL is shown by IFN-γ production response to peptide loaded target cells in the ELISPOT assay.

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