Generating HPV specific T helper cells for the treatment of HPV induced malignancies using TCR gene transfer

Table 3 Functional activity of A9 TCR transgenic CD4+ T cells as determined in a cytokine bead array assay.

Target cells used in this assay were the HLA-A2 matched, non-autologous model tumor cell line melAKR, either or not carrying a minigene encoding for either HPV16E7_(11-20wt) or HPV16E7_(11-20V). TCR transgenic T cells not co-expressing CD8αβ did not produce cytokine above threshold levels (data not shown). The amounts of cytokines produced, as determined in a cytokine bead array, are depicted as pg/ml. Results shown are representative for two different donors tested.

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