Figure 4
Phenotypic analysis and functional activity of HPV16E7 (11-20) specific A9 TCR transgenic CD4+ T cells. (A) CD4+ T cells were transduced with cmTCRα-2A-cmTCRβ (left hand plot) and a proportion was co-transduced with CD8α-2A-CD8β (right hand plot). Cells were analyzed for binding to E7 tetramers and CD8 expression. (B) Functional activity of TCR-A9 transgenic CD4+ T cells as determined in an intracellular IFNγ staining. Effector cells did (black bars) or did not (open bars) carry the CD8αβ construct. Target cells used were HLA-A2 matched, non-autologous JY loaded with 1 μM irrelevant Influenza MP(58-66) or relevant HPV16E7(11-20) peptide; or the HLA-A2 matched non-autologous model tumor cell line melAKR either or not carrying a minigene construct encoding either HPV16E711-20wt or HPV16E711-20V. Results shown in panels A and B are representative for three different donors tested.