Figure 3
Functional activity of HPV16E6 (73-104) specific 24.101 TCR transgenic CD4+ T cells against peptide or protein loaded target cells. (A) IFNγ production of TCR transgenic CD4+ T cells as determined in an intracellular IFNγ assay. Target cells used were HLA-DP1 matched, non autologous, mature dendritic cells exogenously loaded with relevant HPV16E6(73-104) or irrelevant HPV16E6(37-68) peptide. Intracellular IFNγ staining is shown for GFP/NGFR double positive T cells. (B) Functional avidity analysis of HPV16E6(73-104) specific TCR transgenic CD4+ T cells as determined in an intracellular IFNγ assay. Target cells used were the HLA-DP1 positive cell line EBV24 (autologous to the original 24.101 T cells) loaded with serial 10-fold dilutions of the HPV16E6(73-104) peptide. (C) Proliferative capacity of 24.101 TCR transgenic CD4+ T cells as determined in a thymidine incorporation assay. Target cells used were DP1 matched, non-autologous, monocytes loaded overnight with 20 μg/ml relevant E6 or irrelevant flu protein. Results shown in panels A, B and C are representative for three different donors tested.