Figure 2From: Generating HPV specific T helper cells for the treatment of HPV induced malignancies using TCR gene transferTCRVβ17 expression analysis of codon-modified/cysteinized HPV16E6 (73-104) specific 24.101 TCR transgenic CD4+ T cells. Isolated CD4+ T cells transduced with vectors cmCysLZRS-TCRα1-IRES-GFP and cmCysLZRS-TCRβ17-IRES-NGFR were analyzed for GFP and NGFR marker gene expression by flow cytometry. Cells in the GFP/NGFR double negative quadrant (Gate R3) staining positive for TCRVβ17 represent the TCRβ17+ cells endogenously present in this CD4+ population. In de GFP/NGFR double positive gate R4 37% stain with the TCRVβ17 antibodies. About 6.4% represent the endogenously population, hence about 30% of the cells appear to be TCR transgenic. Part (6.4%) of the T cells in gate R5 stained positive with TCRVβ17 antibodies, again representing the endogenously present population. About 18% (24% minus 6.4% endogenous) of the TCRVβ17 positive cells are transgenic for the TCRVβ17-IRES-NGFR construct. Thus clearly showing that the TCRVβ17 was capable of pairing with endogenously present TCRα chains. Results shown here are representative for three different donors tested.Back to article page