Figure 1

Phenotypic analysis and functional activity of wildtype 24.101 TCR specific for HPV16E6 _(73-104) in TCR transgenic Jurkat/MA cells. (A) Jurkat/MA cells transduced with vectors LZRS-wtTCRα-I-GFP and LZRS-wtTCRβ-I-NGFR were analyzed for GFP and NGFR marker gene expression by flow cytometry. Transduced cells co-expressing GFP and NGFR (gate R3) were negative for irrelevant TCRVβ (upper right plot) but positive for relevant TCRVβ17 (lower right plot) staining, as indicated by the percentage TCRβ positive cells (upper right quadrant). (B) Functional activity of 24.101 TCR transgenic Jurkat/MA cells as determined in a luciferase assay in response to stimulation with the HLA-DP1 positive cell line EBV24, exogenously loaded with relevant HPV16E6_(73-104) or irrelevant HPV16E6_(37-68) peptide. Luciferase activity in Jurkat/MA cells is shown in Relative Luminescence Units (RLU), defined as the ratio of luciferase activity in stimulated versus unstimulated cells Data are shown from one representative experiment out of two performed.