Effects of storage on assay sensitivity. Whole blood (Panels A & B) or PBMCs (Panels C & D) were stored for variable periods of time at room temperature (RT), 4°C (whole blood) or -80°C (PBMCs) before stimulation with BZLF1 or PHA for 18 hours followed by RNA extraction. Induction of MIG (Panels A&C) and IP-10 (Panels B&D) mRNA transcripts are shown normalized to the negative control (ACTs). Columns show the mean RQ value and 95% confidence intervals calculated from triplicate wells. Results are representative of three individual experiments.