*30 mice from 3 independent experiments (10 mice in each experimental group) were cervically dislocated on the 15th day after left cardiac ventricle injection of 5 × 104 melanoma cells in 0.1 ml HEPES-buffered DMEM. See "Materials and Methods" section for details.
†Each bone was scored as either containing a metastatic nodule or being free of microscopic tumor, and the percentage of bones positive for metastases was calculated for the total number of bones sites.
The number of recorded metastases per bone segment (maximum of 10) was multiplied by the surface percentage occupied by metastases (maximum of 100) and expressed as a relative percentage with respect to a previously defined maximum for each individual bone segment. Data represent average values ± SD (n = 30).
Paired and multiple bones were considered as single organ sites with the incidence and metastasis development index calculated including both or all the bones within an animal.