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Table 1 Metastasis development in high metastasis incidence bones following Injection of murine B16 melanoma cells into the left cardiac ventricle of mice*

From: Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow microenvironment via tumor cyclooxygenase-2

  Metastasis Average Metastasis
Bones Incidence (%)† Development index
Maxilla 76.1 63.2 ± 4.3
Mandible 77.5 63.2 ± 3.9
Tibia 69.6 51.9 ± 3.5
Femur 74.4 40.6 ± 2.7
Spine 68.4 32.5 ± 2.7
Ribs 72.2 26.5 ± 2.9
Scapula 58.3 35.4 ± 2.0
Humerus 73.5 42.6 ± 3.5
  1. *30 mice from 3 independent experiments (10 mice in each experimental group) were cervically dislocated on the 15th day after left cardiac ventricle injection of 5 × 104 melanoma cells in 0.1 ml HEPES-buffered DMEM. See "Materials and Methods" section for details.
  2. †Each bone was scored as either containing a metastatic nodule or being free of microscopic tumor, and the percentage of bones positive for metastases was calculated for the total number of bones sites.
  3. The number of recorded metastases per bone segment (maximum of 10) was multiplied by the surface percentage occupied by metastases (maximum of 100) and expressed as a relative percentage with respect to a previously defined maximum for each individual bone segment. Data represent average values ± SD (n = 30).
  4. Paired and multiple bones were considered as single organ sites with the incidence and metastasis development index calculated including both or all the bones within an animal.