| | Metastasis | Average Metastasis |
|---|
Bones | Incidence (%)† | Development index |
|---|
Maxilla | 76.1 | 63.2 ± 4.3 |
Mandible | 77.5 | 63.2 ± 3.9 |
Tibia | 69.6 | 51.9 ± 3.5 |
Femur | 74.4 | 40.6 ± 2.7 |
Spine | 68.4 | 32.5 ± 2.7 |
Ribs | 72.2 | 26.5 ± 2.9 |
Scapula | 58.3 | 35.4 ± 2.0 |
Humerus | 73.5 | 42.6 ± 3.5 |
- *30 mice from 3 independent experiments (10 mice in each experimental group) were cervically dislocated on the 15th day after left cardiac ventricle injection of 5 × 104 melanoma cells in 0.1 ml HEPES-buffered DMEM. See "Materials and Methods" section for details.
- †Each bone was scored as either containing a metastatic nodule or being free of microscopic tumor, and the percentage of bones positive for metastases was calculated for the total number of bones sites.
- The number of recorded metastases per bone segment (maximum of 10) was multiplied by the surface percentage occupied by metastases (maximum of 100) and expressed as a relative percentage with respect to a previously defined maximum for each individual bone segment. Data represent average values ± SD (n = 30).
- Paired and multiple bones were considered as single organ sites with the incidence and metastasis development index calculated including both or all the bones within an animal.
