Effect of celecoxib and anti-VEGF on the proliferation rate of BMSC-CM-treated B16M (A) and A375M (B) cells. Murine B16M (A) or A375M (B) cells were plated onto 96-well plates at a density of 2,500 cells per well. Some cells received BMSC-CM, LPS-treated BMSC-CM or 10 ng/ml rmVEGF in the presence or absence of 1 μg/ml anti-VEGF monoclonal antibody or 1 μg/ml celecoxib. Control melanoma cells were cultured in the presence of basal medium (DMEM). After 48 h incubation, the number of cells was determined by microscopic counting in 5 different fields per well and by sulforhodamine-101-based fluorimetry as described in Methods. Every assay was done in quadruplicate and repeated three times. Data represent average values ± SD. Differences were statistically significant cells (P < 0.01) with respect to (*) basal medium- or (**) BMSC-CM- or (#) LPS-treated BMSC-CM or (##) rmVEGF-treated melanoma cells according by ANOVA and Bonferroni's post-hoc test.