Figure 2

Effect of LPS on the metastasis incidence (A) and volume (B) of major bone segments of mice injected in the LCV with B16M cells. Mice (n = 15) were injected intravenously with LPS (0.5 mg/kg body weight). Control mice (n = 15) received the same volume of saline. Six hours later, both mouse groups were LCV-injected with 5 × 10⁴ B16M cells in 0.1 ml HEPES-buffered DMEM as described in Methods. After 15 days all mice were killed by cervical dislocation and the incidence and volume of metastasis were determined using morphometrical procedures. This experiment was repeated three times. Results are expressed as mean increase percentages with respect to metastasis incidence and volume in control mice.