Figure 6

TUNEL assay for indentifying apoptotic nuclei in brain infarct area. The number of apoptotic nuclei (yellow arrows) notably higher in group 2 (B) than in group 1 (A), group 3 (C), group 4 (D) and group 5 (E), significantly higher in group 3 and 4 than in group 1 and 5, and significantly higher in group 5 than in group 1, but no significant difference between group 3 and 4. Scale bars in right lower corner represent 20 μm (400x). † vs. other groups, p < 0.001 (ANOVA test). Symbols (*, †, ‡, §) indicate significance (at 0.05 level) (by Scheffe multiple-comparison post hoc test).