Figure 4

Protein expression levels of inflammation and oxidative stress-related in infarct area. A) and B) Remarkably elevated protein expressions of inducible nitric oxide synthase (iNOS) (A) and NADPH oxidase 1 (NOX-1) (B) in group 2 than in other groups, notably higher in group 3 and 4 than in group 1 and 5, significantly increased in group 5 than in group 1, but no difference between group 3 and 4. † vs. other groups, p < 0.001 (ANOVA test). C) No significant difference in NOX-2 protein expression among all groups. D) Remarkably lower protein expressions of endothelial (e)NOS in group 2 than in other groups, notably lower in group 3 and 4 than in group 1 and 5, but no difference between group 3 and 4. Similar eNOS protein expression noted between group 1 and group 5. † vs. other groups, p < 0.001 (ANOVA test). Symbols (*, †, ‡, §) from A) to D) indicate significance (at 0.05 level) (by Scheffe multiple-comparison post hoc test) (n = 6 for each group).