Figure 5

Effect of LRAST on the frequency of CD4⁺CD25⁺Foxp3⁺ cells. Mice were treated with LRAST or mGC8 GM-CSF/IFA in a therapeutic setting as described in Figure 4A. The mice were killed at day 9 after vaccination and splenocytes were analyzed by flow cytometry for the expression of Treg markers (FoxP3 and CD25). (A) Percentage of FoxP3⁺CD25⁺ cells calculated as a percentage of CD4⁺ T cells. (B) Absolute number of CD4⁺CD25⁺Foxp3⁺ cells calculated from initial splenocyte counts. (C) Ratio of CD8⁺ T cells to CD4⁺CD25⁺Foxp3⁺ (Tregs) and (D) Ratio of CD4⁺ non-Tregs to Tregs. (LRAST, n = 4; mGC8 GM-CSF/IFA, n = 2). Means and SE are indicated.