Figure 2

Immunohistochemical staining against hBD-1 (400-fold magnification). A: BONJ. Increased immunoreactivity can be detected within bone marrow area (BM), mostly in bands along the endosteal cell lines (arrows). Inside mineralized bone trabecula (BT) immunostaining demonstrates nuclear expression of hBD-1. B: ORN. Immunoreactivity is barely visible in cytoplasm of stromal cells. Specific cells for diagnosis of ORN in terms of radicytes (arrows) were traceable. C: Healthy bone samples. Detailed enlargement of mineralized area (BT) of controls shows a Haversian channel (arrow) in which positive nuclear staining is seen only along the endosteal cell line. Immunoreactivity of osteocytes is negative in this sample.