Table 1 Primers and hydrolysis probes used in real-time PCR.
Gene | TaqMan probe No | Forward primer 5'→3' | Reverse primer 5'→3' |
|---|---|---|---|
CGB | #71 Roche Diagnostic, Cat. No: 04688945001 | TACTGCCCCACCATGACC | CACGGCGTAGGAGACCAC |
GNRH1 | #29 Roche Diagnostic, Cat. No: 04687612001 | GACCTGAAAGGAGCTCTGGA | CTTCTGGCCCAATGGATTTA |
HPRT | Human HPRT Gene Assay (Roche Diagnostic, Cat. No: 05046157001) | ||