Inflammation up-regulates the expression of α7nAChR mRNA in the murine colon. A. Samples of colon were collected at 7 days from dextran sulfate sodium (DSS)-treated (n = 5) and control (n = 5) mice and expression of α7nAChR mRNA was determined in preparations of longitudinal muscle with adherent myenteric plexus (LMMP) using real-time reverse transcriptase polymerase chain reaction (RT-PCR). RT-PCR demonstrated α7nAChR mRNA in the LMMP and an increase in its expression by inflammation. Significant up-regulation of IL6 mRNA was also observed while proliferator-activated receptor-γ (PPAR-γ)1 and PPAR-γ2 mRNA levels remained unchanged. B. RT-PCR analysis of α7nAChR, IL6, PPAR-γ1 and PPAR-γ2 mRNA expression in LMMP isolated from the normal and inflamed murine colon. M = markers; Lane 1 = control; Lane 2 = inflamed colon. Unpublished research.