Antibodies generated in mice immunized with dual vaccine, WSN-Aβ
bind to Aβ
and inhibit its neurotoxicity: (A) Sera isolated from WSN-Aβ1-10, but not WSN-WT vaccinated mice at dilution 1:200 bound to all species of Aβ42 peptide, including oligomers recognized by A11 oligomer-specific antibodies. Control monoclonal 6E10 antibody bound to all forms of Aβ42 peptide. (B) Anti-Aβ1-10 inhibits Aβ42 fibrils- and oligomer-mediated toxicity. Human neuroblastoma SH-SY5Y cells were incubated with Aβ42 oligomers and Aβ42 fibrils, in the presence or absence of anti-Aβ1-10 antibody or irrelevant mouse IgG. Control cells were treated with the vehicle, and cell viability was assayed in all cultures using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data were collected in four replicate and was expressed as a percentage of control ± s.d.