Figure 4

Presence of CD31+ and von Willebrand Factor (vWF) Cells in Lung Parenchyma. (Upper Panel) Immunofluorescent (IF) staining (200 x) of CD31+ cells with green color in lung parenchyma. Notably fewer number of CD31+ cells (white arrows) in ischemia-reperfusion (IR) group (B) than in normal control (A) and IR + adipose-derived mesenchymal stem cell (ADMSC) (C) groups. C) Merged picture from double staining (Dil + CD31) showing mixed color of red and yellow cells [under high magnification (a) of the dotted box (b)], indicating implanted CD31-positive cells presented in lung the lung parenchyma. D) *p < 0.01 between the indicated groups. (Lower Panel) IF staining (200 x) of von Willebrand factor (vWF)+ cells with green color in lung parenchyma. Notably reduced number of vWF+ cells (white arrows) in IR group (F) than in normal control (E) and IR + ADMSC (G) groups. G) Merged picture from double staining (Dil + CD31) showing mixed color of red, green, and yellow cells [under high magnification (c) of the dotted box (d)], indicating the presence of implanted vWF-positive cells in lung parenchyma. H) *p < 0.001 between the indicated groups. Symbols (*, †, ‡) indicate significance (at 0.05 level) (by Bonferroni multiple comparison post hoc test). Scale bars in right lower corner represent 50 μm. n = 6 in each group.