Figure 3

Synergy in the antiviral activity of GL along with interferon. GL shows synergistic effect with interferon-α (5 IU/well) against HCV in liver cells (Huh-7). Huh-7 cells were incubated for 6 h with GL and interferon alone, or combination of GL and interferon in a 96-well plate. After 6 h cells were infected with 2 × 10⁴ copies of HCV 3a genotype per well and incubated for additional 18 h. At the end of incubation period, total RNA was extracted by Gentra kit, and the levels of HCV RNA remaining were determined, by real time Quantitative RT-PCR assay and are shown as percentage of HCV RNA survival in cells. Results are represented as the average and standard error for three independent experiments. *P value > 0.05 vs control.