Figure 2

Impact of conditioned medium on cardiac fibroblast gene expressions. Effects of fresh medium (G1), 10% and 20% concentration of normal rat myocardium-derived conditioned medium (MDCM) (G2) and 10% and 20% of myocardial infarct-related MDCM (G3) on gene expressions of neonatal cardiac fibroblasts (n = 6 in each group). (A) mRNA expression of α-smooth muscle actin (SMA). G1 vs. G2 (10% & 20%) vs. G3 (10% & 20%), p < 0.01. Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level) (by Bonferroni multiple comparison post hoc test). (B) & (C) mRNA expressions of both collagen type I α-1 (B) and collagen type I α-2 (C). *p < 0.02 between the indicated groups. (D) mRNA expression of major activator membrane type 1-matrix metalloproteinase (MT1-MMP). *p < 0.01 between the indicated groups. (E) & (F) mRNA expressions of matrix metalloproteinase (MMP)-2 and MMP-9. *p < 0.01 between the indicated groups. (G) mRNA expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). *p < 0.02 between the indicated groups. (H) mRNA expressions of vascular endothelial growth factor (VEGF). *p < 0.01 between the indicated groups. (I) mRNA expressions of vascular endothelial growth factor (VEGF). *p < 0.001 between the indicated groups.