A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

Table 7 Filtered Dataset and Detection Rate

MULTIMER	Donor	Dump Channel	Median (filtered)	Detection Rate
CMV pp65	1	No	0.11	16 (80%)
		Yes	0.10	17 (85%)
	2*	No	n.a.	0 (0%)
		Yes	n.a.	0 (0%)
	3	No	0.17	18 (90%)
		Yes	0.14	20 (100%)
	4	No	0.08	17 (85%)
		Yes	0.06	18 (90%)
Melan-A	1	No	0.18	10 (50%)
		Yes	0.16	12 (60%)
	2	No	0.23	9 (45%)
		Yes	0.18	12 (60%)
	3	No	0.10	10 (50%)
		Yes	0.09	10 (50%)
	4	No	0.06	5 (25%)
		Yes	0.04	5 (25%)

Filtered results obtained using two MULTIMERS in four donors stratified by use of a DUMP channel. For all sixteen experimental conditions the (i) the median of the reported values from experiments with a positive response in both conditions (filtered), and (ii) response detection rates are displayed. The asterisk indicates a negative control donor.

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