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Table 6 Data Filter 2 - Visual Confirmation

From: A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

    Sum of Dot Plot Evaluation Score*
Antigen Donor Dump Channel 0 1 2 3 4
CMV p65 1 No 0 0 1 3 16
   Yes 0 0 2 3 16
  2 No 20 0 0 4 0
   Yes 19 1 0 8 0
  3 No 0 0 1 0 19
   Yes 0 0 0 0 20
  4 No 0 1 1 1 16
   Yes 0 1 1 1 17
Melan-A 1 No 3 2 5 2 10
   Yes 3 1 4 0 12
  2 No 4 2 5 2 8
   Yes 4 0 4 0 12
  3 No 5 1 4 1 9
   Yes 1 2 5 3 11
  4 No 8 3 3 6 6
   Yes 7 1 7 5 4
  1. Filter 2: Visual Confirmation from Dot Plot Evaluation. The reported dot plots were assessed by a central review of all the dot plots. A dot plot was assigned a score of "0" when there was clearly no clustered population (or the experiment was not performed or not interpretable), a score of "1" when the clustering was ambiguous and a score of "2" when there was clearly a clustered population. The sum of the scores for each duplicate is presented in the table. The columns in bold indicate experiments that did not meet the optical evaluation criteria (< = 2) and therefore were not considered a positive response.