A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

Table 5 Data Filter 1 - Reproducibility

			Percent Difference between Duplicates
Antigen	Donor	Dump Channel	0-10%	10-30%	30-200%	> 200%*
CMV p65	1	No	9	3	5	3
		Yes	9	5	3	3
	2	No	6	6	4	4
		Yes	4	5	3	8
	3	No	13	5	2	0
		Yes	9	9	2	0
	4	No	9	9	1	1
		Yes	3	10	6	1
Melan-A	1	No	8	3	7	2
		Yes	6	11	3	0
	2	No	7	5	6	2
		Yes	7	8	5	0
	3	No	7	7	5	1
		Yes	5	10	2	3
	4	No	8	4	2	6
		Yes	5	5	5	5

Filter 1: Reproducibility, Based on Percent Difference. The datasets were grouped by the variation of reported MULITMER-positive frequencies in staining duplicates. Duplicates that showed high variation (> 200%) were not considered as a positive response and are indicated in bold. *This group also includes duplicates with missing data, namely only one staining was performed.