A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

Table 4 %age of Irrelevant-MULTIMER-positive CD8-positive events

MULTIMER	Donor	Dump Channel	Dead Cell Staining	N	Median
Irrelevant	1	No	No	6	0.04	↓
		No	Yes	14	0.02
		Yes	No	6	0.04	↓
		Yes	Yes	14	0.01
	2	No	No	6	0.06	↓
		No	Yes	14	0.03
		Yes	No	6	0.05	↓
		Yes	Yes	14	0.02
	3	No	No	6	0.04	↓
		No	Yes	14	0.03
		Yes	No	6	0.02	↓
		Yes	Yes	14	0.02
	4	No	No	6	0.03	↓
		No	Yes	14	0.02
		Yes	No	6	0.03	↓
		Yes	Yes	14	0.01

Results obtained using the irrelevant MULTIMERS in four donors stratified by DUMP channel use and further subdivision by the use of dead cell marker. The table also indicates the number of labs (N) for each of the 16 subgroups. The table further indicates the median values of the reported percentages of MULTIMER+ CD8+ cells for all reported data sets using the irrelevant MULTIMER. Arrows in both tables denote decreased values when a DUMP channel is used.

ISSN: 1479-5876