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Table 4 %age of Irrelevant-MULTIMER-positive CD8-positive events

From: A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

MULTIMER Donor Dump Channel Dead Cell Staining N Median  
Irrelevant 1 No No 6 0.04
   No Yes 14 0.02  
   Yes No 6 0.04
   Yes Yes 14 0.01  
  2 No No 6 0.06
   No Yes 14 0.03  
   Yes No 6 0.05
   Yes Yes 14 0.02  
  3 No No 6 0.04
   No Yes 14 0.03  
   Yes No 6 0.02
   Yes Yes 14 0.02  
  4 No No 6 0.03
   No Yes 14 0.02  
   Yes No 6 0.03
   Yes Yes 14 0.01  
  1. Results obtained using the irrelevant MULTIMERS in four donors stratified by DUMP channel use and further subdivision by the use of dead cell marker. The table also indicates the number of labs (N) for each of the 16 subgroups. The table further indicates the median values of the reported percentages of MULTIMER+ CD8+ cells for all reported data sets using the irrelevant MULTIMER. Arrows in both tables denote decreased values when a DUMP channel is used.