Skip to main content

Table 3 %age of CMV pp65- and Melan-A-MULTIMER-positive CD8-positive events

From: A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

MULTIMER

Donor

Dump Channel

Median(raw)

 

CMV pp65

1

No

0.12

↓

  

Yes

0.10

 
 

2

No

0.04*

↓

  

Yes

0.02*

 
 

3

No

0.17

↓

  

Yes

0.14

 
 

4

No

0.08

↓

  

Yes

0.07

 

Melan-A

1

No

0.17

↓

  

Yes

0.09

 
 

2

No

0.24

↓

  

Yes

0.18

 
 

3

No

0.10

↓

  

Yes

0.08

 
 

4

No

0.06

↓

  

Yes

0.04

 
  1. The medians of the reported percentages of MULTIMER-positive CD8-positive cells for each antigen-donor combination are shown in the table. These results are stratified by condition (with and without the inclusion of a dump channel). Results obtained using two MULTIMERS in four donors stratified by use of a DUMP channel. For all sixteen experimental conditions the median of the reported values for MULTIMER+ CD8+ cells for all experiments are displayed. The asterisk indicates a negative control donor.