Figure 2

Representative comparative spectratype analysis for BV families found in spleens, pancreatic lymph nodes and pancreas from untreated and B-cell reconstituted NOD.Igμ^null mice. Splenocytes (SP) and Pancreatic lymph nodes (PLN) were analyzed from naïve NOD.Igμ^null and B cell-reconstituted mice (NOD.Igμ^null + B cells). Gaussian profiles for BV2, BV10, BV12 and BV14 families were found in spleens and lymph nodes. Pancreata (PN) of naïve NOD.Igμ^null animals had no expansions for these clonotypes, but non-gaussian expansions were detected in high frequency following B cell reconstitution.