Figure 3

Oxidative damage of ADSC. ROS are measured using H₂DCF-DA staining of ADSC and quantification in a flow cytometer (A). NO concentration was measured using standard Griess reagent (B). Expression level of glutathione dismutase gene was measured using qRT-PCR (C). The level of oxidative damage was investigated using ELISA in young and aged ADSC (normoxia and hypoxia) (D). Results are means of 5 different animals and considered significantly changed when: * = p < 0.05, ** = p < 0.01, *** = p < 0.001.