Immunohistochemical staining of MMP-1 from the OE-33 adenocarcinoma cancer cell line and MMP-1 gene expression in human BE and EAC. MMP-1 staining in cytospins from the OE-33 adenocarcinoma cancer cell line served as additional positive control (left) and showed 65% positive cells; IgG control (right) (a). Gene expression of MMP-1 in human BE and EAC. MMP-1 gene expression in BE was significantly (p = 0.01) lower in comparison to EAC without BE. Normal tissue is considered as one-fold (b). Statistically significant value is indicated with an asterisk.