Figure 5
From: S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis)
Comparison of the effects of S110 and decitabine on DNA methylation of the γ-globin gene promoter region in baboons. Red rectangles = methylated CpG; green rectangles = unmethylated CpG, yellow rectangles = polymorphic sites where no CpG dinucleotides are present. Results are expressed as the % deoxymethylcytosine (dmC) of cytosines located within CpG dinucleotides at positions -54, -51, +5, +16, and +48 with respect to the transcriptional start site of the baboon γ-globin gene promoter. Each row corresponds to the sequence analysis of an individual cloned PCR product derived from bisulfite-treated DNA. Results at each CpG site (-54, -51, +5, +16, +48) are within each corresponding column.