Figure 3
From: S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis)
Comparison of the effects of S110 and decitabine on DNA methylation of the γ-globin gene promoter region in cultured human erythroid progenitor cells. The effects of decitabine and S110 on the DNA methylation of 5 CpG sites located within the 5' γ-globin promoter region are shown. Red rectangles = methylated CpG; green rectangles = unmethylated CpG. Results are expressed as the % deoxymethylcytosine (dmC) of cytosines located within CpG dinucleotides at positions -54, -51, +5, +16, and +48 with respect to the transcriptional start site of the human γ-globin gene promoter. Each row corresponds to the sequence analysis of an individual cloned PCR product derived from bisulfite-treated DNA. Results for each CpG site (-54, -51, +5, +16, +48) are in each corresponding column.