Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma

Table 3 Gene expression comparison of normal epithelium versus tumor cells, and normal stroma versus tumor stroma

Case	Sample	Detector	Avg.Ct	St. Dev	ΔCtN	Sample	Av.Ct	St. dev	ΔCtT	ΔΔCt	2^{^}-ΔΔCt
1	NE	MMP3	39.59		17.86	TE	25.50	0.108	5.37	-12.48	↑
	NE	MMP10	38.59	3.43	16.85	TE	21.39	0.07	1.25	-15.59	↑
	NE	ACTB	21.73	0.22		TE	20.13	0.03
	NS	MMP3	36.20	0.75	15.77	TS	28.79	0.19	9.3	-6.47	↑
	NS	MMP10	35.38	0.75	14.95	TS	22.35	0.07	2.85	-12.09	↑
	NS	ACTB	20.43	0.06		TS	19.49	0.04
2	NE	MMP3	39.54		13.77	TE	39.87		12.29	-1.48	↑
	NE	MMP10	38.68		12.92	TE	30.25	0.02	2.66	-10.25	↑
	NE	ACTB	25.76	0.166		TE	27.58	0.22
	NS	MMP3	39.97		15.03	TS	32.58	0.42	4.21	-10.82	↑
	NS	MMP10	UD			TS	31.02	0.17	2.65	OFF-N, ON-T	↑
	NS	ACTB	24.93	0.051		TS	28.36	0.159
3	NE	MMP3	UD			TE	38.51	1.49	14.13	OFF-N, ON-T	↑
	NE	MMP10	UD			TE	31.93	0.257	7.55	OFF-N, ON-T	↑
	NE	ACTB	23.45	0.058		TE	24.38	0.07
	NS	MMP3	UD			TS	36.23	1.1	11.44	OFF-N, ON-T	↑
	NS	MMP10	UD			TS	34.13	0.80	9.34	OFF-N, ON-T	↑
	NS	ACTB	24.40	0.016		TS	24.79	0.07
4	NE	MMP3	UD			TE	31.89	0.174		OFF-N, ON-T	↑
	NE	MMP10	39.42		15.99	TE	27.00	0.03	4.59	-11.40	↑
	NE	ACTB	23.43	0.056		TE	22.41	0.033
	NS	MMP3	38.95		15.91	TS	29.61	0.108	5.24	-10.66	↑
	NS	MMP10	39.1		16.06	TS	27.69	0.118	3.32	-12.73	↑
	NS	ACTB	23.03	0.095		TS	24.36	0.05
5	NE	MMP3	UD			TE	29.94	0.172		OFF-N, ON-T	↑
	NE	MMP10	UD			TE	30.28	0.209		OFF-N, ON-T	↑
	NE	ACTB	25.31	0.073		TE	25.08	0.048
	NS	MMP3	39.65		16.22	TS	32.59	0.334	4.81	-11.40	↑
	NS	MMP10	39.38		15.95	TS	33.01	0.451	5.23	-10.72	↑
	NS	ACTB	23.43	0.0013		TS	27.78	0.0911

NE, normal epithelium; NS, normal stroma; TE, tumor epithelium; TS, tumor stroma.

ISSN: 1479-5876