Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation

Table 3 Recoveries of 3d mDC and 7d mDC after cryopreservation and thawing

	3d mDC - EP*		7d mDC - EP*		3d mDC + EP*		7d mDC + EP*
	counts (× 10⁶)	%	counts (× 10⁶)	%	counts (× 10⁶)	%	counts (× 10⁶)	%
Donor 1
Before EP*					2.0	100	2.0	100
Before freezing	1.6	100	1.2	100	1.4	70	1.3	65
After thawing	0.9	59	0.9	73	0.9	43	0.5	26
Donor 2
Before EP*					1.5	100	1.5	100
Before freezing	1.2	100	0.5	100	1.0	65	0.3	23
After thawing	1.0	83	0.5	98	0.7	47	0.3	17
Donor 3
Before EP*					2.0	100	2.0	100
Before freezing	1.4	100	0.7	100	0.5	25	0.5	25
After thawing	0.5	37	0.2	23	0.2	11	0.1	3
Donor 4
Before EP*					1.5	100	1.5	100
Before freezing	1.1	100	0.7	100	1.4	93	0.7	45
After thawing	1.0	91	0.6	86	0.9	61	0.4	27
mean % after thawing ± SD⁺		68 ± 24		70 ± 33		41 ± 21		18 ± 11

ISSN: 1479-5876