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Table 3 Recoveries of 3d mDC and 7d mDC after cryopreservation and thawing

From: Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation

  3d mDC - EP* 7d mDC - EP* 3d mDC + EP* 7d mDC + EP*
  counts
(× 106)
% counts
(× 106)
% counts
(× 106)
% counts
(× 106)
%
Donor 1         
Before EP*      2.0 100 2.0 100
Before freezing 1.6 100 1.2 100 1.4 70 1.3 65
After thawing 0.9 59 0.9 73 0.9 43 0.5 26
Donor 2         
Before EP*      1.5 100 1.5 100
Before freezing 1.2 100 0.5 100 1.0 65 0.3 23
After thawing 1.0 83 0.5 98 0.7 47 0.3 17
Donor 3         
Before EP*      2.0 100 2.0 100
Before freezing 1.4 100 0.7 100 0.5 25 0.5 25
After thawing 0.5 37 0.2 23 0.2 11 0.1 3
Donor 4         
Before EP*      1.5 100 1.5 100
Before freezing 1.1 100 0.7 100 1.4 93 0.7 45
After thawing 1.0 91 0.6 86 0.9 61 0.4 27
mean % after thawing ± SD+   68 ± 24   70 ± 33   41 ± 21   18 ± 11
  1. * EP: electroporation
  2. + SD: standard deviation