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Table 1 Yield, purity and viability of immature and mature DC

From: Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation

 

2d iDC

6d iDC

3d mDC

7d mDC

Donor 1

    

Yield*

n.d.+

n.d.+

8%

4%

Purity #

n.d.+

n.d.+

39%

34%

Viability $

n.d.+

n.d.+

94%

78%

Donor 3 &

    

Yield*

12%§

6%§

18%

9%

Purity#

57% 

58% 

60%

70%

Viability$

93% 

84% 

95%

86%

Donor 4 &

    

Yield*

n.d.+

n.d.+

4%

3%

Purity#

32% 

30% 

42%

60%

Viability$

85% 

76% 

86%

81%

  1. * Yield: from PBMC with the starting population set at 100%.
  2. + n.d.: not determined
  3. # Purity: SSC/FSC
  4. $ Viability: propidium iodide (PI) stain
  5. &Donors 3 and 4 are identical with donors 3 and 4 in Table 2 and Table 3.
  6. § These values are lower compared to 3d and 7d mDC due to cell loss from strong adherence of iDC.