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Table 1 Yield, purity and viability of immature and mature DC

From: Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation

  2d iDC 6d iDC 3d mDC 7d mDC
Donor 1     
Yield* n.d.+ n.d.+ 8% 4%
Purity # n.d.+ n.d.+ 39% 34%
Viability $ n.d.+ n.d.+ 94% 78%
Donor 3 &     
Yield* 12%§ 6%§ 18% 9%
Purity# 57%  58%  60% 70%
Viability$ 93%  84%  95% 86%
Donor 4 &     
Yield* n.d.+ n.d.+ 4% 3%
Purity# 32%  30%  42% 60%
Viability$ 85%  76%  86% 81%
  1. * Yield: from PBMC with the starting population set at 100%.
  2. + n.d.: not determined
  3. # Purity: SSC/FSC
  4. $ Viability: propidium iodide (PI) stain
  5. &Donors 3 and 4 are identical with donors 3 and 4 in Table 2 and Table 3.
  6. § These values are lower compared to 3d and 7d mDC due to cell loss from strong adherence of iDC.