Figure 7

Small Arteriolar Density Analysis and Cardiac Hypotrophic Gene Expression. α-SMA immunohistochemical staining (A-E) (200×) for the number of small arterioles (≤ 25 μm in diameter) in LV myocardium on day 90 after DCM induction. The results showing notably lower small vessel (red arrows) number in DCM group than in other groups. F) * p < 0.0001 between the indicated groups. Scale bars in right lower corner represent 50 μm (n = 8 in each group). The mRNA expressions of α-myosin heavy chain (MHC) (G) and β-MHC of LV myocardium on day 90 after DCM induction. G) * p < 0.003 between the indicated groups. H) * p < 0.0003 between the indicated groups. All symbols (*, †, ‡, §, ¶) in F), G) and H) indicate significant difference (at 0.05 level) by Tukey multiple comparison procedure (n = 8 in each group).