Figure 3

CD40+ Cell Expression in LV Myocardium and Intensity of Oxidative Stress. Immunohistochemical staining (400×) (A-E) for identifying CD40-positive cells (red arrows) in LV myocardium on day 90 following DCM induction (n = 8 in each group). F) * p < 0.0001 between the indicated groups. Symbols (*, †, ‡, ¶) indicate significant difference (at 0.05 level) by Tukey multiple comparison procedure. Scale bars in right lower corner represent 20 μm. Western blotting results (G) of oxidative index, protein carbonyls, in LV myocardium on day 90 following DCM induction (upper panel), with quantification results of each group (n = 8) (lower panel). * p < 0.0003 between the indicated groups. Symbols (*, †, ‡, ¶) indicate significant difference (at 0.05 level) by Tukey multiple comparison procedure. Note: Right lane and left lane shown on upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively.