Figure 2
Apoptosis and Fibrosis in LV Myocardium. TUNEL assay (400×) of apoptotic nuclei (A-E) (red arrows) of LV myocardium on day 90 following DCM induction (n = 8). F) * p < 0.0001 between the indicated groups. Symbols (*, †, ‡) indicate significant difference (at 0.05 level) by Tukey multiple comparison procedure. Scale bars in right lower corner represent 20 μm. Mean fibrotic area (μm2)/high-power field (HPF) (200×) in each group (n = 8) of rats on day 90 following DCM induction. Masson's trichrome stain (G-K) demonstrating markedly increased fibrosis area (yellow arrows) in DCM group compared to other groups. L) * p < 0.001 between the indicated groups. Symbols (*, †, ‡, §, ¶) indicate significant difference (at 0.05 level) by Tukey multiple comparison procedure. Scale bars in right lower corner represent 50 μm.