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Table 3 Results of discordant case investigation between OSNA and histology (H&E Staining and CK19 IHC

From: One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas

No. Stage
UICC
Histology OSNA qRT-PCR Conclusion
   size description original run copies/μL +/- second run1copies/μL +/- beta-actin
+/-
CK19
+/-
CEA
+/-
 
1 IV 8 mm Macrometastasis, only in slice b, not d <250 - 5470 ++ + + + Discordant
Sample mix-up
2 III m Macrometastasis, calcified tissue <250 - 0 - + + - Discordant
Beta-actin and CK19 value near the cut-off level
3 IV 3 mm Macrometastasis, only level 2 <250 - <250 - + - - Tissue allocation bias
4 IIA - negative 540 + <250 - + - - Discordant
Low copy
5 IIA - negative 1300 + <250 - + - - Discordant
6 IV   negative 1300 + 290 + + - + Tissue allocation bias
7 IIIA 3.5 mm Positive with IHC in level 1 25000 ++ 53700 ++ + + + Tissue allocation bias
8 IB - negative 27000 ++ <250 - + - - Discordant
Sample mix-up
  1. OSNA: ++: CK19 mRNA copies/μL higher than 5000, +:CK19 mRNA between 250 - 5000 copies/μL, -: CK 19 mRNA copies/μL less than 250, 1The indicated copy number is the mean of three OSNA runs, QRT-PCR: +: positive, -: negative.