Figure 5

Immunohistochemical staining of MHC. Reddish-brown immunoreactivity of class I and II MHC were shown in panel A and B, respectively. A representative coronal section of the hippocampus of an ischemic rat brain without injection of NGF or NSC exhibited no positivity of class I MHC (A-i). Intense staining of class I MHC was noted in the cytoplasm of pyramidal neurons in the hippocampus of ischemic rats undergone neural stem cell transplant for four weeks (A-ii). Clusters of cells with class I MHC-positivity were evident in the infarcted brain parenchyma of transplanted rats (A-iii and A-iv). A comparable extent of class II MHC was noted in ischemic rats irrespective of any therapy but unremarkable in normal rat (panel B, top row). Reddish-brown staining of Class II MHC was evident in the infarcted brain parenchyma (B-i), along the meninge (B-ii), areas near the ventricular lining and vascular wall near the hippocampus (B-iv) of transplanted rats. Scale bars: 75 μm